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The fatty acids used in the production of E471 can be derived from both vegetable and animal fats. Common sources include palm oil, soybean oil, and canola oil. The manufacturing process typically involves a procedure known as glycerolysis, where glycerol reacts with fatty acids to form mono- and diglycerides. The end product is usually considered safe for consumption and is generally recognized as safe (GRAS) by food safety authorities in many countries.

Acids work primarily by lowering the pH of food products. Most bacteria, yeasts, and molds thrive in neutral to slightly alkaline conditions. When the pH is lowered through the addition of acids, it creates an environment that is inhospitable for microbial growth. For instance, many pathogens become inactive or die below a certain pH level, making acidic foods safer for consumption.

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Formic acid can be synthesized through various methods, with one of the most notable being the hydrolysis of methanol carbon monoxide (CO and H2O in the presence of a catalyst). Additionally, it can be naturally produced by certain ants, which is where it derives its name (from the Latin formica, meaning ant) and in the fragmentation of biological materials. Its natural occurrence in plants and animals underscores its importance in biological processes.

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