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Macromolecular oxidation was detected in proteins by the colorimetric measurement of Advanced Oxidation Protein Products (AOPP) and in lipids by the colorimetric quantification of malondialdehyde (MDA). Standard curves were run with chloramine-T and 1,1,3,3 tetraethoxypropane (TEP) for AOPP and MDA methods, respectively [29], [30], [31]. Values were normalized to initial protein content in samples, measured with Bradford reagent [32]. The standard deviation of at least six measures was calculated and p-value < 0.05 were considered significant.

In a study published in 2022 in the journal Particle and Fibre Technology, researchers examined the impact of maternal exposure to titanium dioxide nanoparticles in newborn offspring mice. They found that “a chronic exposure to TiO2 NPs during pregnancy alters the respiratory activity of offspring, characterized by an abnormally elevated rate of breathing.” Breathing was also shown to be “significantly and abnormally accelerated,” and the ability for neural circuitry to effectively adjust breathing rates was impaired. The researchers concluded: “Our findings thus demonstrate that a maternal exposure to TiO2 NPs during pregnancy affects the normal development and operation of the respiratory centers in progeny.”

Lithopone is produced by coprecipitation of barium sulfate and zinc sulfide. Most commonly coprecipitation is effected by combining equimolar amounts of zinc sulfate and barium sulfide: