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A few non-dietary studies have reported adverse effects in the gastrointestinal tract of laboratory animals given food-grade TiO₂. However, these same effects were not seen when the same or higher doses of food-grade TiO₂ were administered in the animals' diet. Dietary studies best reflect how humans are exposed to TiO₂ from food. Thus, the Food Directorate placed the most emphasis on the results of these studies in the state of the science report.

The morphology of vitaminB2@P25TiO₂NPs is coherent with the description of Degussa P25 typical population. Size distribution histograms were made from manual measures of the nanoparticles observed in SEM micrographs using ImageJ®. This data showed that more than 70% is anatase (between 20 and 60 nm) with a minor amount of rutile characteristic bars (between 80 and 100 nm) and a small amount of amorphous phase (<40 nm) [36]. Further analysis of the same sample areas with an EDS probe demonstrated the presence of organic material composed of C and O (Fig. 2). This material was found homogeneously distributed on the surface of the different shapes of P25TiO₂NP, not in the background, indicating a specific interaction that could be attributed to the functionalization of the P25TiO₂NPs with vitamin B2.

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Titanium dioxide can form several different shapes, which have different properties. Some shapes can be converted to nanomaterials. Micronized TiO2 (also called “nano” or “nanoparticles”) was introduced in the early 1990s.Nanotechnology and micronization both refer to the practice of creating very small particles sizes of a given material. “Nanoparticles” usually refers to particles smaller than 100 nanometers; a nanometer is 1/1 billionth of a meter. At these small sizes, and at low concentrations, titanium dioxide appears transparent, allowing for effective sunscreens that do not appear white.