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The analysis of the supernatant by UV–Vis spectrometry showed that each gram of P25TiO₂NP is loaded with 0.17 g of vitamin B2, after washing them. This value is coherent with the thermogram (Fig. 1), which showed a loss of 19% of weight, attributed to the thermal decomposition of vitamin B2.

The US and Canada, however, approve the use of titanium dioxide as a food additive. Canada's recent review of titanium dioxide reconfirmed its safety and pointed out that many of the toxicity studies the EU reviewed were not relevant to the safety of titanium dioxide as a food ingredient, and that the ban is based on an abundance of caution and uncertainty.

Here it has been shown that functionalization of P25TiO2NPs with vitamin B2 was able to significantly decrease the oxidative stress produced when they are exposed to sunlight. This finding is of main importance to prevent skin damage and toxicity of sunscreens containing this form of untreated titanium dioxide and should be taken into consideration when updating the regulations mentioned above .